11/2/2022 0 Comments Palindromic sequence![]() Palindromes and near-palindromes also serve as binding sites for restriction enzymes and transcription factors (TFs). Because of this tendency, they are often responsible for chromosomal translocations, recombinations, and deletions and are associated with the inheritance of many genetic diseases. Palindromic AT-rich repeats (PATRRs) can cause DNA to denature more readily due to weak A–T bonds, thereby increasing the propensity of the sequence to fold into a secondary structure 11. They can alter the DNA replication process and inhibit gene expression by inhibiting ribosomal translocation along the mRNA transcript 9, 10. The sites of cruciform formation are hot spots for DNA breakage and chromosomal translocation 8. A long palindrome has the unique ability to fold back onto itself to form a secondary structure called a cruciform or hairpin (Fig. Short palindromes, under 50 bp, prevent DNA degradation, while palindromes longer than 50 bp result in mutations and DNA instability 6. They stimulate deletions during DNA replication and interchromosomal recombination between homologous sequences, leading to the loss of intervening sequences in fact, palindromes and near-palindromes account for 83% of deletions and small insertions 4, 5. Palindromes affect various cellular processes, including gene expression, regulation, and gene replication 4. Measuring the abundance, frequency, and location of palindromes across the human genome is critical to understanding their functional roles. Palindromic DNA sequences are prevalent in the genomes of a wide variety of organisms, including humans the functions and implications of such sequences are only beginning to be understood. Sequences that are palindromic except for a few mismatches in base pairing are called near-palindromes. If the complementary portions are separated by a gap sequence, the sequence is referred to as inverse repeat 3. When a palindromic sequence is folded at its midpoint, the base pairs (bp) on the two halves are complementary. 1, the sequence on the positive strand 5′-GACA|TGTC-3′ is a palindrome since GACA is followed by its complement CTGT, but appearing in reverse order as TGTC 2. In DNA, palindromes are defined as a sequence of nucleotides that are followed by its complement sequence appearing in reverse order 1. The catalog of palindromes in the reference genome and 1000 Genomes is being made available here with details on their variations in each individual genome to serve as a resource for future and retrospective whole-genome studies identifying statistically significant palindrome variations associated with diseases or traits and their roles in disease mechanisms. The analysis of disease/trait-associated single-nucleotide polymorphisms in palindromic regions showed that disease-associated risk variants are 14 times more likely to be present in palindromic regions than in other regions. We found that ~30% of the palindromes exhibit variation, some of which are caused by rare variants. In this work, we computed and analyzed the palindromic sequences in the human genome and studied their conservation in personal genomes using 1000 Genomes data. Palindromic mutations are linked to many human diseases, such as neuronal disorders, mental retardation, and various cancers. Palindromes are distributed throughout the human genome and play significant roles in gene expression and regulation. Cutting both strands of DNA harms the virus more than cutting one strand.A palindrome in DNA is like a palindrome in language, but when read backwards, it is a complement of the forward sequence effectively, the two halves of a sequence complement each other from its midpoint like in a double strand of DNA. Bacteria have evolved to 'disable' bacteria-targeting viruses (bacteriophages) using restriction enzymes that bind to these palindromic sequences. The last reason has been important in the struggle between viruses and bacteria. It is even possible that two enzymes work as a dimer to cut the palindromic sequence, further increasing efficiency. This means that the enzyme recognizes the sequence no matter from which side the enzyme approaches the DNA.Ī palindromic sequence also increases the chance that both strands of DNA are cut. A palindromic sequence is the same backwards and forwards on both sides (see image below). Luckily! because you don't want a 'pacman' that cuts DNA at random places.ĭNA is double stranded, so it has 'two sides' to which the enzyme can bind. ![]() It binds to the DNA only in one specific configuration. Enzymes such as restriction enzymes have to recognize a very specific sequence in order to carry out its task. ![]()
0 Comments
11/1/2022 0 Comments Alfred 4 license code free![]() ![]() Find important files, open them, copy a paragraph, paste it somewhere else, open a new location, translate a test. Once you've completed your search or task, Alfred 4 can complete other tasks with its search results. With this feature, you can create all kinds of personalized tasks to use with web browsers, specific everyday actions, system items, and more. For example, you could write ‘open Twitter’ and the program will take you right to your Twitter profile. /rebates/&252falfred-powerpack-license-code-free. This saves time when using web browsers or navigating through files.Īnother one of this app's many features is the ability to create personalized commands. state) act code college name city state 5904 advanced placement exams 6291 associated exam board a-levels 5900 clep exams 6292 east africa a-levels 8500 foreign college/university 5901 higher level i.b. Simply enter a keyword and Alfred 4 will search your system, Google, Amazon, or Wikipedia. After that, a search bar will appear near the top of the screen whenever you use the shortcut. You can choose from a variety of housing options ranging from our academic or program-based living learning communities or traditional lifestyle options such as quiet study or substance free. With Alfred 4 you can search both your system and the web, as well as create personalized actions and custom commands that will make your life much easier.Īfter installing Alfred 4, the first thing you’ll have to do is create a keyboard shortcut to start the program. The Alfred State College campus includes 14 residence halls with corridor, suite and apartment style living options. This program can noticeably increase your productivity and make almost any task easier. With the VS Code extension you get practically zen mode, search for the necessary snippets and insert them immediately or save the selected code sections as a snippet. ![]() ![]() Alfred 4 is a tool that almost everyone should use. massCode supports extensions for VS Code, Raycast and Alfred, which gives even more possibilities to use application. ![]() 11/1/2022 0 Comments Astroboard with bluetooth![]() ![]() Key Features: Bluetooth 5.0 | 2 in 1 Wireless transmitter with receiver | aptX Low latency | 3.5mm Audio jack connection | 33ft connectivity range | Rechargeable internal battery | Up to 10 hours battery backup | Trond Bluetooth Transmitter ![]()
11/1/2022 0 Comments Mac cammask![]() If you’re regularly using online meetings across many services, Man圜am is probably worth the annual fee or pay for a lifetime licence. Handy for quizzes or countdown for a break in an online meeting. Many other options including onscreen clock, timer and stopwatch options. MAC CAMMASK PLUSManycam Studio ($59 a year or $119 lifetime) includes blur and custom video background plus support for two devices. Here we’ve made three presets a side by side view of camera and PowerPoint slide, Picture in Picture (mostly slide with live camera in the corner) and an ‘I’ll be back’ choice for when you have to leave for a short time. You can setup multiple preset video feeds and switch between them whenever you like during a call. It has a wide range of options including support for multiple cameras, desktop capture, split screen, picture in picture in their Standard plan ($29 a year). MAC CAMMASK ANDROIDIt’s available for Windows, Mac, iPhone, iPad and Android devices. Man圜am is the ‘big daddy’ in virtual cameras. If you’ve had experience with any of these, we’d love to hear from you Man圜am We’ve not had time to test them but hope to fully review some in the near future. as a virtual background or ‘picture in picture’. Live computer desktop or window can be used as a background or as a video feed.Some are limited to only physical cameras.Accepts any video feed including from a smartphone or tablet webcam, IP camera.Apple and Android devices would be great.There are various virtual camera options available and we’re focusing on these options Turning your smartphone into a webcam – iPhone, iPad or Android – these tools send your devices video to Windows or Mac and shows up as another camera in the video devices list.SnapCamera is mostly playful and funny additions to a video.If you’ve been following Office Watch’s coverage of Remote Working and online meetings, you’ll have already seen some virtual cameras: No need to change the settings in each meeting software, just choose the ‘virtual camera’ and you’re away. MAC CAMMASK SOFTWAREWelcome to the Virtual Camera … a piece of software that takes a real camera feed, lets you change it (blur, virtual background etc.) then select that as a ‘camera’ in Zoom, Teams, Meet or whatever. One camera feed with consistent selection of virtual backgrounds and other effects all would be great, and it is possible. Currently we have Zoom, Teams, Skype and Google Meet all setup. Most of us use different video conferencing options depending on what the host has chosen. What would be ideal is a way to setup those features with your webcam then use that video feed on whichever calling software you like. Other online calling software either don’t have them or they are configured in different ways. Teams is getting them with some limitations. Zoom has blur and virtual background options. You can have a blurred or virtual background for any online video call, not just ones that have those in-built features. ![]() 11/1/2022 0 Comments Anaconda 3![]() ![]() ![]() Then once it was discovered the anacondas were eating these things and living forever, one of the bad actors could've deadpanned, "What are these things? Bananacondas?" BWAHAHAHAHA! Man, I really need to start writing screenplays for intentionally bad movies. My biggest complaint is that Anacondas wasn't cheesy ENOUGH! If you're gonna be bad, then go as far out on the cheesy limb as you can go! Instead of a blood orchid, their research should've discovered a rare, Indonesian banana that held the secret to long life. However, this one does have more anacondas! It's been 7 years since I saw the original, but I'm pretty sure it's the better of the two. ANACONDA 3 MOVIEThe first movie had famous people in it, this one doesn't. If you're wondering how this compares to the original Anaconda, well, the two really have nothing to do with each other except for presence of an anaconda. It's funny up to a point, but eventually you start looking at your watch and thinking, "OK snake, it's time to eat this kid." The CGI looks a little goofy at times, but most of it is well done thanks to the wise decision to hide the flaws with darkness and water. We're treated to such intellectually-challenged exchanges as: "What's wrong with this picture?" "You're in it." And it's mostly dominated by Messner's Michael Paré-esque line delivery and Eugene Byrd's banshee-like screeching. And since this is a "creature feature," by definition a lot of the action takes place at night, in dark caves and in water, with only flashlights to light the way. In other words, you can expect a whole lot of stuff like a door opening slowly and then something jumping out while a loud noise is made. This is one of those movies that relies on every cliché in the B-movie book. ![]() When B-movie characters like her prance around with a huge chip on their shoulder, I usually pray for some sort of creature to sneak up and bite that chip right off. My first choice was the Jennifer Lopez wannabe. She eventually was only accenting about one word per sentence, but by the climax she started fakin' it up with reckless abandon! And I'm sure plenty of girls will think Johnny "Five O'Clock Shadow" Messner is pretty hot, but for the most part you'll spend the first 10 minutes of the movie picking which characters you want to see swallowed whole by a giant anaconda. But why do producers insist on accents being so fake? Her accent dwindled as the movie went along. My money says you really won't care about anybody involved in this production. He died soon enough, so it doesn't really matter. He looked like Gregg Rainwater from The Young Riders. Some other guy of some sort of foreign descent was thrown in there as well. But hey, we've got everything else!" And that, my friends, is your cast. Now how about somebody who's actually famous?" "Um, well, we don't exactly have that. He's in wardrobe right now having the sleeves on his tight shirt rolled up." "Perfect. And I saw that you found a white European male, but how about the protagonist?" "Oh, you mean the muscular white guy with a 2-day beard growth and husky voice? Check. She's from Georgia, but her accent sounded too realistic. ![]() How about a blonde babe with a thick, fake Southern accent?" "Check. That'll alert everybody that he's not one of those brainy Asians. It wasn't easy, but we found one with a mullet." "Perfect. Did y'all find that tough-looking Asian guy?" "Check. How about a Latino woman with an unnecessarily bad attitude?" "You mean the J-Lo wannabe? Check." "Good. Who is Morris Chestnut?" "Well, we thought it was a brand of chewing tobacco, but he's actually who we're gonna use as our tough black guy." "Oh, OK. Don't wanna be accused of stereotyping." "Good idea. But let's put a tough black dude in there as well. All right, I've kept this secret, but I actually sat in on a production meeting for Anacondas and here's how it went down: "Annoying black dude who just screams the whole time?" "Check. Bring the crackers because mama, this movie is bringin' the cheese! If there has ever been a more culturally diverse group of no-name actors to brave the jungles of Borneo then I'll end my movie reviewing career right now (future users of the Alan Smithee pseudonym applaud). ![]() 11/1/2022 0 Comments Dedalus and icarus![]() And to this day, when summer breezes blow and the wildflowers bloom, the voice of Perdix may still sometimes be heard, calling to his mate from among the grass and forest trees. While he was falling through the air, she changed him into a partridge, and he flew away to the hills to live forever in the woods and fields which he loved so well. ![]() Poor Perdix fell headlong through the air, and he would have fatally landed upon the stones at the foot of the cliff had not kind Athena seen him and taken pity upon him. Then, when the boy obeyed, it was easy enough, with a strike of a hammer, to knock the scaffold down.Ī grey partridge. One morning when the two were putting up a decoration on the outer wall of Athena’s temple, Daedalus told his nephew to go out on a narrow scaffold which hung high over the edge of the rocky cliff where the temple stood. “If he keeps on in this way,” he whispered to himself, “he will be a greater man than me his name will be remembered, and mine will be forgotten.” Athena helping Perdix, by Crispijn van de (I) Passe, 1602-1607, $\ccpd$ĭay after day, while at his work, Daedalus reflected over this matter, and soon his heart was filled with hatred towards young Perdix. Then he invented the wheel which potters use to shape clay, and he made of a forked stick the first pair of compasses for drawing circles, and he studied out many other intriguing and useful things.ĭaedalus was not pleased when he saw that the boy was so skilled and wise, so ready to learn, and so eager to do anything. Dedalus and icarus how to#Seeing how a certain bird carved holes in the trunks of trees, he learned how to make and use the chisel. Walking one day by the sea, he picked up the spine of a large fish, and from it he invented the saw. His eyes were ever open to see what was going on about him, and he learned everything about the fields and the woods. But Perdix was a very quick learner and soon surpassed his uncle in the knowledge of many things. ![]() He built a stone palace for Aegeus, the young king of Athens, and renovated the Temple of Athena which stood on the great rocky hill in the middle of the city.ĭaedalus had a nephew named Perdix whom he had taken as a boy to be his apprentice. He invented many things that helped many people. He was the first to attach things together with glue. It was he who taught the people how to build better houses and how to hang their doors on hinges and how to support the roofs with pillars. While Athens was still only a small city there lived within its walls a man named Daedalus who was the most skillful worker in wood and stone and metal that had ever been known. Unit 4: Hubris and Nemesis art by ReyeD33 on Deviantart, $\ccbyncnd$ Daedalus, The Wonderful ArtisanĪdapted from Old Greek Stories by James Baldwin, $\ccpd$ ![]() |
AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |